High Performance Liquid Chromatography

Principle

In liquid chromatography, a liquid is used as the mobile phase, and it analyzes compounds in a mixture.This analytical technique is generally used for separating components in a mixture based on the interaction properties of analytes to both mobile and stationary phase. The high performance liquid chromatography (HPLC) rapidly separates the components in a mixed sample, as the pump drives the mobile phase liquid and the sample through a column, packed with a suitable solid material used as a stationary phase, and then detects each component by the detector. Detectors include UV-visible absorptiometric detector, differential refractometer, evaporative light scattering detector…etc. BY selecting columns and detectors, quantitative analysis of various organic compounds (non-volatile) is possible.

Applications

• Quantitative analysis of pesticides, aldehydes and surfactants
• Quantitative analysis of active ingredients in food and medicine
  　(i.e., quercetin, glycyrrhizinic acid, ginsenoside, -carotene, berberine…etc.)
• Quantitative analysis of additives
  　(i.e., thiourea, benzotriazole, aminotetrazole, melamine, sodium saccharin, imidazole…etc.)

Important

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